Technical Data
B2765-03A
Brk (Protein Tyrosine Kinase 6, PTK6)
Description:
PTK6 (protein tyrosine kinase 6, BRK or FLJ42088), a 451-amino acid protein of about 5kD, encodes a cytoplasmic nonreceptor protein kinase that may function as an intracellular signal transducer in epithelial tissues. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation. It is present at very high levels in the colon, small intestine, and prostate, and at low levels in some fetal tissues. It is expressed at low levels in some breast tumors, but not in normal breast tissue. It is also found in melanocytes, but it is not expressed in the heart, brain, placenta, lungs, liver, skeletal muscle, kidney, or pancreas. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8A245Affinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Ni-NTA purified truncated recombinant PTK6 expressed in E. coli strain BL21 (DE3)
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, with 0.02% sodium azide.
Specificity:
Recognizes truncated PTK6 recombinant protein / MCF-7 cell lysate (WB), and PTK6 recombinant protein (ELISA).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.