Technical Data
B2788-45C
Bu-1 (PE)
Description:
Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. Recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.1-2

Applications:
Flow cytometry 2
Immunoprecipitation 2
Immunohistochemistry (acetone-fixed, frozen tissue sections)

Recommended Dilution:
Flow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k5K98Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceChickenMouse
Concentration:
~0.1mg/ml
Immunogen:
Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with PE.
Specificity:
Recognizes chicken Bu-1 (chB6), Mr 70-75kD. Does not crossreact with turkey cells when analyzed by immunofluorescent staining and flow cytometry.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tregaskes, C.A., N. Bumstead, T.F. Davison, and J.R. Young. 1996. Immunogenetics 44(3):212-217.
2. Rothwell, C.J., K. Vervelde, and T.F. Davison. 1996. Vet. Immunol. Immunopathol. 55(1-3):225-234.