Technical Data
B2788-50
Bu-1a
Description:
Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.

Applications:
• Flow cytometry 1
• Immunoprecipitation 1
• Immunohistochemistry (frozen sections)

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k5K93Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceChickenMouse
Concentration:
~0.5mg/ml
Immunogen:
Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens
Purity:
Purified
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Specificity:
Recognizes chicken Bu-1a alloantigen, Mr 70kD. Does not react with cells from CHA and H.B15 strains by immunofluorescence.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Veroma, T., O. Vainio, E. Eerola, and P. Toivanen. 1988. Hybridoma 7:41.
2. Houssaint, E., E. Diez, and J.R. Pink. 1987. Immunol. 62:463.