Technical Data
B2788-50D
BU-1A (Bu1a, B6.1)
Description:
Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:200 -1:1,000; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B337Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceChickenMouse
Concentration:
~1mg/ml
Immunogen:
Chicken Bursa cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes the 'a' allele of the polymorphic cell surface antigen Bu-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos. Eur. J. Immunol. 19: 239-243.