Technical Data
B2788-50D1
BU-1A (Bu1a, B6.1) (FITC)
Description:
Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20-1:80; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG110B337Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceChickenMouse
Concentration:
~0.1mg/ml
Immunogen:
Chicken Bursa cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes the 'a' allele of the polymorphic cell surface antigen Bu-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos. Eur. J. Immunol. 19: 239-243.