Technical Data
B2790-40
BubR1 (Bub1b, Bub1A, MAD3L, budding uninhibited by benzimidazoles related 1, budding uninhibited by benzimidazoles 1 homolog beta, mitotic arrest deficient-like 3 like)
Description:
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2500-1:7500. Whole cell lysate (50ug for Input, 1mg for IP) from HeLa cells that were untreated (-, interphase) or treated with Nocodazole (+, mitotic). Antibody used at 0.2ug/ml for Western Blot and at 3ug/mg lysate for IP.
Immunoprecipitation: 1-5ug/mg lysate. Immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Region between residues 350 and 400 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in TrEMBL entry Q8WV50 (GeneID 701).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human BubR1. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.