Technical Data
B2790-40C
BUBR1, NT (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1 beta, MAD3/BUB1-related Protein Kinase, hBUBR1, Mitotic Checkpoint Kinase MAD3L, Protein SSK1, BUB1B, MAD3L, SSK1)
Description:
BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:200
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human BUB1B (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human BUB1B.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Cahill, D.P., et al., Genomics 58(2):181-187 (1999). 2.Davenport, J.W., et al., Genomics 55(1):113-117 (1999). 3.Chan, G.K., et al., J. Cell Biol. 143(1):49-63 (1998). 4.Cahill, D.P., et al., Nature 392(6673):300-303 (1998). 5.Taylor, S.S., et al., J. Cell Biol. 142(1):1-11 (1998).