Technical Data
B2850-01
Bromodeoxyuridine (BrdU)
Description:
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Fluorescent In Situ Hybridization (FISH) Assay. Other applications not tested.

Recommended Dilutions:
Western Blot: Band is observed at ~307kD
Immunohistochemistry: Frozen and paraffin sections. Enzymatic antigen retrieval and DNA acid denaturation required.
Fluorescent In Situ Hybridization: 1:25; Use a rabbit anti-sheep AMCA secondary antibody.
Note: Denature DNA sample first so base is accessible to BrdU antibody.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceSheep
Concentration:
~1mg/ml
Immunogen:
Bromodeoxyuridine, conjugated to HGG (Human Gamma Globulin)
Purity:
Purified by Protein G affinity chromatography followed by polishing chromatography against resin (HGG).
Form
Supplied as a liquid in PBS, pH 7.6. No preservative added.
Specificity:
Recognizes Bromodeoxyuridine at ~307kD. Species Crossreactivity: all species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Goldstein, B.G. et al., (2007) Am J Physiol Gastrointest Liver Physiol 292: G1784-G1792. 2. Tetreault, M. (2010) Gastroenterology 139; 2124-2134.e9. 3. Martin, C.D. (2007) Neurosci Lett. 427:148-152. 4. Katz, J.P. et al., (2005) Gastroenterology 128:935-945 (IH). 5. Crissey, M.A.S. et al., (2008) Neoplasia 10: 8-19. 6. Soleimanpour, S.A. et al., (2010) J. Biol. Chem. 285; 40050-40059. 1. Akino, K., et al., Wound Repair Regen. 11(5): 354-360 (2003). 2. Hoshino, T., et al., Cytometry 6: 627-632 (1985). 3. Campana, D., et al., Journal of Immunological Methods 107(1): 79-88 (1988).