Technical Data
B2850-01B
Bromodeoxyuridine (BrdU)
Description:
BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry.
Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:200. Use 20ul to label 10e6 cells in 100ul
Immunohistochemistry (Paraffin): 1:25-1:200
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-49H IgG, F(ab)2, H&L X-Adsorbed (HRP) RbxRt
I1904-49U IgG, F(ab)2, H&L X-Adsorbed (FITC) RbxRt

Recommended Negative Controls:
I1904-83M1: IgG2a, Rat Negative Control

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3H579Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceRat
Concentration:
~1mg/ml
Immunogen:
Bromodeoxyuridine (BrdU)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6: 501-505. 2. Ghiringelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J. Exp. Med. 202: 919-929. 3. Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27: 339-369.