Technical Data
B2850-01E
Bromodeoxyuridine (BrdU)
Description:
BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating DNA.

Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen, paraffin): 1:5-1:10 Dilute in 0.15M PBS, 1% BSA, 1% sodium-azide. Requires protease digestion of paraffin sections.
Immunocytochemistry: Pulse labeling of dividing cells will allow the immunocytochemical identification of S-phase cells.
Optimal dilutions to be determined by the researcher.

Positive Control:
Immunohistochemistry: Bromodeoxyuridine labeled cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1IIb5Ascites
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
BrdU (BSA)
Purity:
Ascites
Form
Supplied as a liquid, 0.02% sodium azide.
Specificity:
Recognizes DNA in which BrdU has been incorporated. Reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. Recognizes BrdU in denaturated (single stranded) DNA. 100% crossreactive with Jodo-Deoxy-Uridine (IrdU).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schutte, B., et al., 1987, J. of Histochemistry and Cytochemistry 35, 371-374. Schutte, B., et al., 1987, J. of Histochemistry and Cytochemistry 35, 1343-1345 2. Schutte, B., et al., 1987, Cytometry 8, 372-376.