Technical Data
B2850-01G
Bromodeoxyuridine (BrdU)
Description:
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgG2bAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceSheep
Concentration:
~1mg/ml
Immunogen:
Highly purified Bromodeoxyuridine, conjugated to KLH.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS. No preservative added.
Specificity:
Recognizes bromodeoxyuridine at ~307kD. Very low crossreactivity to 5-methyl cytosine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hoshino, T., et al., Cytometry 6: 627-632 (1985). 2. Campana, D., et al., Journal of Immunological Methods 107(1): 79-88 (1988).