Description:
BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Applications:
Suitable for use in Flow cytometry. Other applications have not been tested.
Recommended Dilutions:
Flow cytometry: Neat. Use 20ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | BU1/75 (ICR1) | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Rat |
|
| Concentration: |
| ~0.1mg/ml |
| Immunogen: |
| Bromodeoxyuridine (BrdU) |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Suppplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin. |
| Specificity: |
| Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not cross react with thymidine but does react weakly with chlorodeoxyuridine. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6: 501-505. 2. Ghiringelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J. Exp. Med. 202: 919-929. 3. Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27: 339-369.
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