Technical Data
B2850-02B
Bromodeoxyuridine (BrdU)
Description:
BrdU, IdU and CldU are analogs of thymidine, they can incorporate into DNA during DNA synthesis replacing thymidine.Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004)

This antibody has been used to detect CldU to study the speed of DNA replication fork (Bugler et al. 2010) , in the detection of CldU label retaining stem cells (Kimoto et al. 2008) and label retaining neurons (Murata et al. 2011).

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:200; 20ul labels 10e6 cells in 100ul.
Immunohistochemistry (paraffin): 1:25-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a7E101Affinity Purified
SizeStorageShippingSourceHost
250ugBlue IceRat
Concentration:
~0.5mg/ml
Immunogen:
Bromodeoxyuridine (BrdU)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 25% glycerol.
Specificity:
Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Species crossreactivity: Axolotl Cross reacts with chlorodeoxyuridine (CldU) but does not cross react with thymidine or iododeoxyuridine (Aten et al. 1992)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6: 501-505. 2. Ghiringelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J. Exp. Med. 202: 919-929. 3. Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27: 339-369. 4. Das, G. et al. (2009) Cyclin D1 fine-tunes the neurogenic output of embryonic retinal progenitor cells. Neural Dev. 4: 15. 5. Nakhai, H. et al. (2008) Conditional ablation of Notch signaling in pancreatic development.Development. 135: 2757-65.6. Ghai, K. et al. (2010) Notch signaling influences neuroprotective and proliferative properties of mature Müller glia. J Neurosci. 30: 3101-12.7. Amador-Arjona, A. et al. (2011)Primary cilia regulate proliferation of amplifying progenitors in adult hippocampus: implications for learning and memory.J Neurosci. 31: 9933-44.9. Bugler, B. et al. (2010) Unscheduled expression of CDC25B in S-phase leads to replicative stress and DNA damage.Mol Cancer. 9: 29.10. Gonzalo-Gobernado, R. et al (2009) Mobilization of neural stem cells and generation of new neurons in 6-OHDA-lesioned rats by intracerebroventricular infusion of liver growth factor. J Histochem Cytochem. 57: 491-502 11. Xu, Q. et al. (2010) Sonic hedgehog signaling confers ventral telencephalic progenitors with distinct cortical interneuron fates. Neuron. 65: 328-40.12. Zhang, J. et al. (2010) A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons. BMC Neurosci. 11: 158. 13. Bonzo, J.A. et al. (2012) Suppression of Hepatocyte Proliferation by Hepatocyte Nuclear Factor 4a in Adult Mice.
Biol Chem. Jan 12. [Epub ahead of print]