BrdU, IdU and CldU are analogs of thymidine, they can incorporate into DNA during DNA synthesis replacing thymidine.Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004)
This antibody has been used to detect CldU to study the speed of DNA replication fork (Bugler et al. 2010) , in the detection of CldU label retaining stem cells (Kimoto et al. 2008) and label retaining neurons (Murata et al. 2011).
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Flow Cytometry: 1:25-1:200; 20ul labels 10e6 cells in 100ul.
Immunohistochemistry (paraffin): 1:25-1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 0.09% sodium azide, 25% glycerol.|
|Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Species crossreactivity: AxolotlCross reacts with chlorodeoxyuridine (CldU) but does not cross react with thymidine or iododeoxyuridine (Aten et al. 1992)|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
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Biol Chem. Jan 12. [Epub ahead of print]