Technical Data
B2850-02C
Bromodeoxyuridine (BrdU)
Description:
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Applications:
Suitable for use in Flow Cytometry, Immunohistology. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 20ul labels 10e6 cells in 100ul, 1/20
Immunohistology: Neat-1/20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a7E101Highly Purified
SizeStorageShippingSourceHost
5mlBlue IceRat
Concentration:
~1mg/ml
Immunogen:
Bromodeoxyuridine (BrdU).
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.