Technical Data
B2850-02E
Bromodeoxyuridine (BrdU)
Description:
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:25-1:200. Use 20ul to label 10e6 cells in 100ul.
Immunohistochemistry (Paraffin): 1:25-1:200
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagents:
I1904-49H IgG, F(ab)2, H&L X-Adsorbed (HRP) RbxRt
I1904-49U IgG, F(ab)2, H&L X-Adsorbed (FITC) RbxRt

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a7E101Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceRat
Concentration:
~0.5mg/ml
Immunogen:
Bromodeoxyuridine (BrdU).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.01% sodium azide, 25% glycerol.
Specificity:
Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.