Technical Data
Bromodeoxyuridine (BrdU)
Suitable for use in In situ-hybridization, Flow Cytometry and Immunohistochemistry (Frozen and paraffin sections)

Recommended Dilution:
Immunohistochemistry: 1:10; Incubation Time 1 hr at room temperature.

It can be used for:
1. Radioimmunocytochemical detection of circulating levels of BrdU
2 Detection of S-phase cells in tissue sections by immunoperoxidase or immunofluorescence method
3. Detection of S-phase cells in cell suspension
4. Determination of the percentage of profilerating cells by flow cytometry
5. Quantitative determination of the number of various phases of the cell cycle by dual parameter flow-cytometrical analysis

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml-20CBlue IceMouse
Not determined
BrdU conjugated to BSA
Supplied as a liquid.
Reacts with BrdU in denatured (single-stranded) DNA). The antibody is crossreactive with iododeoxyuridine. Species Crossreactivity: All species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Schutte B et al. Effect of Tissue Fixation on Anti-Bromodeoxyuridine Immunohistochemistry. The Journal of Immunohistochemistry and Cytochemistry, Vol 35, 11:1343-1345 (1987)
Schutte B et al. Studies with Anti-Bromodeoxyuridine Antibodies: II. Simultaneous Immunocytochemical Detection of Antigen Expression and DNA Synthesis by in Vivo Labeling of Mouse Intestinal Mucosa. The Journal of Histochemistry and Cytochemistry, Vol 35, 3:371-374 (1987)
Schutte B et al: Studies with Anti-Bromodeoxyuridine Antibodies: I An improved method for the immunocytochemical detection of BrdfU labeled nuclei using flow cytometry. Cytometry 8:372-376 (1987)