Technical Data
B2850-07D
Bromodeoxyuridine (BrdU)
Description:
BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2ug/10e5 cells in 100ul
Immunohistochemistry: Formalin fixed paraffin embedded
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kBu20aAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human BrdU.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human BrdU.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Magaud, J-P., et al. 1989. J. Histochem. Cytochem. 37:1517. 2. Tomihari, M., et al. 2010. Cancer Res. 70:5778. 3. Gratzner, H.G. 1982. Science 218:474. 4. Dolbeare, F., et al 1983. Proc. Natl. Acad. Sci. USA 80:5573. 5. Dolbeare, F., et al. 1985. Cytometry 6:521.