Technical Data
Bromodeoxyuridine (BrdU) (BSA & Azide Free)
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry(frozen & formalin/paraf n):1ug/ml for 30 minutes at RT.
For staining of formalin- xed tissues, incubate sections in 4N HCl for 30 minutes at RT, followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37ΊC

Optimal dilutions to be determined by the researcher.

Positive Control:
Cultured cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Cellular Localization:

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG10.N.35Affinity Purified
100ug4°C (-20°C Glycerol)Blue IceMouse
Bromodeoxyuridine (BrdU) labeled with BSA.
Purified by Protein G affinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.4 Also available with BSA and azide. See B2850-07.
Recognizes free BrdU, BrdU coupled to protein carrier, BrdU in ssDNA (partially denatured dsDNA). Species Reactivity: All species
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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