Technical Data
B2850-10G
Bromodeoxyuridine (BrdU)
Description:
Bromodoxyuridine (BrdU) can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen, paraffin). Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B320Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Bromodeoxyuridine (BSA).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes the thymidine analog bromodoxyuridine (BrdU).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Magaud, J.P. et al. (1989) Double Immunocytochemical labelling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem.37:1517-1527.