Technical Data
B2850-13
Bromodeoxyuridine (BrdU)
Description:
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analog BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Applications:
Suitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100-1:200; Acid methanol treatment required.
Immunohistochemistry (frozen and paraffin-embedded sections with acid/proteinase treatments): 1:100-1:200
Optimal dilutions to be determined by the researcher.

Note: In order for the antibody to work a pretreatment to expose DNA will be needed (1mg/ml of pepsin in 0.01M HCl for 30 min at RT).

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q390Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouse
Concentration:
~1mg/ml
Immunogen:
A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to BSA.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schutte, B., et al., Cytometry 8: 372376 (1987). 2. Tinnemans, M.M.F.J., et al., Br. J. Cancer 67: 1217-1222 (1993).