Technical Data
B2850-14
Bromodeoxyuridine (BrdU)
Description:
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identifying proliferating cells in blood (1), tissues (2,3), tumors (4–7), as well as for determining plasma cell labeling indices (8).
TypeIsotypeCloneGrade
MabIgG12Q2284Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Bromodeoxyuridine BSA conjugate
Purity:
Purified immunoglobulin
Form
Liquid in 10mM Phosphate buffer, pH 7.4 containing 150mM sodium chloride and 0.1% sodium azide.
Specificity:
Binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.