Technical Data
Bromodeoxyuridine (BrdU)
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:3 dilution of this lot showed positive immunostaining for BrdU in HeLa cells xed with 95% ethanol, 5% glacial acetic acid. Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.

Optimal dilutions to be determined by the researcher.

Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20C. May be stored at 4C for short-term only. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
4x2.5ml-20CBlue IceMouse
5-iodouridine:ovalbumin conjugate.
Supplied as 4 vials. Each vial contains 2.5ul lyophilized mouse culture supernatant. Reconstitute each vial with 500ul of water containing 0.1% azide.
Recognizes 5-Bromo-2-deoxyuridine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gonchoroff, N.J., et. al., Cytometry; 6: 506512, 1985.
2. Gonchoroff, N.J., et. al., J. Immunol. Methods; 93: 97101, 1986.
3. Greipp, P.R., et. al., Mayo Clin. Proc; 62: 969977, 1987.
4. Beauregard, P., et. al., Hematol Pathol; 5: 17783, 1991.