Suitable for use in ELISA. Other applications not tested.
ELISA: 1:1,000 (Dilute the conjugate with phosphate buffered saline containing 0.05%)
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.|
|Reacts with bromodeoxyuridine (Brdu) and crossreacts with iododeoxyuridine (IdU). Will react with BrdU incorporated into cells of any species. Detection of incorporated Brdu is useful in identifying cells in the S phase of the cell cycle, which may be important in studying the roles of various factors in cell cycle regulation. Crossreactivity: tissue samples of all species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Khan, S et al: J Surg Oncol 39:114,1988 2. Shibui, S et al: J Histochem Cytochem 7:1007,1989 3. Raza,A et al: Leuk Res 11:1079, 1987 4. Miller,MA et al: J Histochem Cytochem 39: 407,1991|