Technical Data
B2850-24C
Bromodeoxyuridine (BrdU)
Description:
5-bromodeoxyuridine (BrdU) is an analogue of thymi- dine. When incubated with tissue, proliferating cells incorporate BrdU into the DNA during S phase and this provides an accurate means of determining cell prolifer- ation. This antibody detects incorporated BrdU in cells or tissues.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:200-1:400 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Cells or tissues with incorporated Brdu, nuclear staining pattern

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG185-2C8Supernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Conjugate of BrdU and helix pomatia haemocyanin.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Specificity:
Recognizes Bromodeoxyuridine. Species Crossreactivity: Any species with incorporated BrdU.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Acta Histochemica, Supplement. Band XXXVI. 353-359 (1988). 2.Raza A, Ucar K and Preisler H D. Double labelling and in vitro versus in vivo incorporation of bromodeoxyuridine in patients with acute nonlymphatic leukaemia. Cytometry. 6:633-640 (1985). 3.Raza A, Preisler H D, Mayers G L, et al.. Rapid enumeration of S-phase cells by means of monoclonal antibodies. New England Journal of Medicine. 310(15):991 (1984). 4.Gratzner H G. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science. 218:474-475 (1982).