Technical Data
B2852-48
Bromodomain and PHD Finger Containing 1 (BRPF1, BR140)
Description:
The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2-2ug/ml
Immunoprecipitation: 100-500ug
Flow Cytometry: 0.5-2ug
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18C42Affinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Partial sequence of recombinant full-length protein to human Bromodomain and PHD Finger Containing 1
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human recombinant Bromodomain and PHD Finger Containing 1 at ~40kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.