Technical Data
B2852-83A
BRD4, NT (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1)
Description:
BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. The gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human BRD4 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human BRD4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Gagnon, D. et al. J Virol. May; 83(9): 4127-4139(2009). 2.Maruyama, T., et al., Mol. Cell. Biol. 22(18):6509-6520 (2002). 3.French, C.A., et al., Am. J. Pathol. 159(6):1987-1992 (2001). 4.Dey, A., et al., Mol. Cell. Biol. 20(17):6537-6549 (2000). 5.David Gagnon, et al. J. Virol., Feb 2009; 10.1128/JVI.02468-08. (Applications: WB)(E-pub)