		Technical Data
B2852-83A	BRD4, NT (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1)

Description:
BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. The gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human BRD4 (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human BRD4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Gagnon, D. et al. J Virol. May; 83(9): 4127-4139(2009). 2.Maruyama, T., et al., Mol. Cell. Biol. 22(18):6509-6520 (2002). 3.French, C.A., et al., Am. J. Pathol. 159(6):1987-1992 (2001). 4.Dey, A., et al., Mol. Cell. Biol. 20(17):6537-6549 (2000). 5.David Gagnon, et al. J. Virol., Feb 2009; 10.1128/JVI.02468-08. (Applications: WB)(E-pub)