Technical Data
Media
Brucella Agar (Powder)
B2900
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone10.0
Yeast Extract2.0
Sodium Chloride5.0
Sodium Bisulfite0.1
Peptic Digest of Animal Tissue10.0
Dextrose1.0
Agar15.0
Total:43g/liter
Cultivation of fastidious bacteria including Brucella sp.

Description:
Brucella Agar is a prepared medium for the cultivation of Brucella sp. from clinical and dairy products. With the addition of blood and menadione, Brucella Agar can be used to cultivate anaerobic bacteria. Cultivation of Brucella sp. requires CO2 enriched

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions per Liter Suspend 43 grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). To prepare blood plates, cool medium to 45- 50.0C and add aseptically 5% sheep red blood or 10% horse blood. Dispense into sterile Petri plates.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Microbiological Analysis: Brucella abortus ATCC 4315

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Yellow beige, slightty turbid.

pH (4.3% @ 25C): 7.00.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.