Technical Data
Media
Brucella Broth (Powder)
B2900-02
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone10.0
Dextrose1.0
Peptic Digest of Animal Tissue10.0
Sodium Chloride5.0
Sodium Bisulfite0.1
Yeast Extract2.0
Total:28g/liter
Cultivation of fastidious bacteria including Brucella sp.

Description:
Brucella Broth is a prepared medium for the cultivation of Brucella sp. from clinical and dairy products. With the addition of blood and menadione, Brucella Broth can be used to cultivate anaerobic bacteria. Cultivation of Brucella sp. requires CO2 enriched

Storage:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions: Mix 28.0g per liter of ddH2O until evenly dispersed. Heat with stirring if necessary to dissolve completely. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Brucella ovis

Appearance: Light beige, homogeneous, free flowing, powder.

Solubility: Yellow beige, slightty turbid.

pH (2.8% solution at 25C): As reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.