Technical Data
B5001-60
BTLA (B and T Lymphocyte Attenuator, CD272)
Description:
B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kD type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V-type Ig-like domain, which interacts with the Herpes virus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.

Applications:
Suitable for use in Western Blot and Direct ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~2ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a7H92Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRat
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived recombinant human BTLA (aa25-150) Accession # Q7Z6A9
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ull sterile PBS.
Specificity:
Recognizes human BTLA. Does not crossreact with recombinant mouse BTLA or recombinant human HVEM.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.