Technical Data
B5001-60C
BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743)
Description:
B- and T-lymphocyte attenuator (BTLA) is a type I transmembrane glycoprotein which is structurally and functionally related to cytotoxic T lymphocyte antigen-4 (CTLA-4), and programmed death-1 (PD-1). BTLA is a T-cell inhibitory receptor that regulates T cell activation through its interaction with herpes virus entry mediator (HVEM), a co-stimulator receptor of the TNFR family. BTLA may negatively regulate T-cell dependent autoimmune reaction, and polymorphisms in its gene have been associated with Rheumatoid Arthritis.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:20-1:200; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from C3H/HeN mice.

Negative Control:
Human tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a10B1178Affinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
BTLA-transfected L cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human B- and T-lymphocyte attenuator (BTLA).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Otsuki N et. al., Biochem Biophys Res Commin 344:1121-1127. 2. Lin C. et al., J Biomed. Sci. 13: 853-860