Technical Data
B5001-60C1
BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743)
Description:
B- and T-lymphocyte attenuator (BTLA) is a type I transmembrane glycoprotein which is structurally and functionally related to cytotoxic T lymphocyte antigen-4 (CTLA-4), and programmed death-1 (PD-1). BTLA is a T-cell inhibitory receptor that regulates T cell activation through its interaction with herpes virus entry mediator (HVEM), a co-stimulator receptor of the TNFR family. BTLA may negatively regulate T-cell dependent autoimmune reaction, and polymorphisms in its gene have been associated with Rheumatoid Arthritis.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from C3H/HeN mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a10B1179Affinity Purified
SizeStorageShippingSourceHost
100ug4C/-20C (No Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
BTLA-transfected L cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human B- and T-lymphocyte attenuator (BTLA).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.