		Technical Data
B5001-60C1	BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743)

Description:
B- and T-lymphocyte attenuator (BTLA) is a type I transmembrane glycoprotein which is structurally and functionally related to cytotoxic T lymphocyte antigen-4 (CTLA-4), and programmed death-1 (PD-1). BTLA is a T-cell inhibitory receptor that regulates T cell activation through its interaction with herpes virus entry mediator (HVEM), a co-stimulator receptor of the TNFR family. BTLA may negatively regulate T-cell dependent autoimmune reaction, and polymorphisms in its gene have been associated with Rheumatoid Arthritis.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from C3H/HeN mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	10B1179	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C/-20°C (No Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

BTLA-transfected L cells.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes human B- and T-lymphocyte attenuator (BTLA).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.