Technical Data
B5001-61C
BTLA (B and T lymphocyte associated, BTLA1, CD272, FLJ16065, MGC129743, B and T lymphocyte attenuator) (PE)
Description:
BTLA, is a 70kD type I membrane glycoprotein that belongs to the CD28 family of receptors. CD28 receptors are Ig superfamily proteins, with an extracellular IgV-like domain, that function as co-stimulatory or co-inhibitory T cell signaling molecules. BTLA is expressed on B cells, T cells, and dendritic cells. Binding of BTLA to its ligand, Herpes virus entry mediator (HVEM, TNFRSF14) triggers a potent inhibitory signal that blocks T cell activation. The aa sequence of the extracellular domain of BTLA shares only 34% identity with that of mouse BTLA. A human BTLA variant lacking the transmembrane domain has been reported.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption: 488nm

Emission: 565-605nm

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceMouseGoat
Concentration:
As reported
Immunogen:
Recombinant human B and T Lymphocyte Attenuator (rhBTLA; aa5-150 with V105M, S138G, and M148V substitutions) expressed in NSO cells (Q7Z6A9).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes mouse BTLA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.