		Technical Data
B5001-61C	BTLA (B and T lymphocyte associated, BTLA1, CD272, FLJ16065, MGC129743, B and T lymphocyte attenuator) (PE)

Description:
BTLA, is a 70kD type I membrane glycoprotein that belongs to the CD28 family of receptors. CD28 receptors are Ig superfamily proteins, with an extracellular IgV-like domain, that function as co-stimulatory or co-inhibitory T cell signaling molecules. BTLA is expressed on B cells, T cells, and dendritic cells. Binding of BTLA to its ligand, herpesvirus entry mediator (HVEM, TNFRSF14) triggers a potent inhibitory signal that blocks T cell activation. The aa sequence of the extracellular domain of BTLA shares only 34% identity with that of mouse BTLA. A human BTLA variant lacking the transmembrane domain has been reported.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption: 488nm

Emission: 565-605nm

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C Do Not Freeze	Blue Ice	Mouse	Goat

Concentration:

As reported

Immunogen:

Recombinant human B and T Lymphocyte Attenuator (rhBTLA; aa5-150 with V105M, S138G, and M148V substitutions) expressed in NSO cells (Q7Z6A9).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

Specificity:

Recognizes mouse BTLA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.