Technical Data
B5001-61P
BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743)
Description:
Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270 (HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. CD272 levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines (1). Polymorphism of the CD272 molecule has been linked to rheumatoid arthritis (3).

Applications:
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k11K2Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to soluble human CD272
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
Specificity:
Recognizes human CD272. Binds to CD272 on the cell surface of human PBMC in Flow cytometry and to recombinant CD272-muIg in EIA. Blocks binding of Biotinylated CD270 (HVEM)-muIg to CD272-muIg in EIA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Derre’ L, DE Speiser, et al. (2010) J Clin Invest 120(1):157-167, 2. Otsuki N, Azuma M, et al. (2006) Biochem Biophys Res Com 344(4): 1121-1127, 3. Lin SC, Chan CH, et al. (2006) J Biomed Sci 13(6): 853-860, 4. Pasero C, D Olive, et al. (2009) Curr Mol Med 9(7): 911-927, 5. Gavrieli M, KM Murphy, et al. (2006) Adv Immunol 92: 152-185.