Technical Data
Media
Buffered Peptone Water (Powder)
B8910
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/liter
Pancreatic Digest of Gelatin10.0
Sodium Chloride5.0
Disodium Phosphate1.5
Monopotassium Phosphate3.5
Total:20g/liter
Helps in recovery of Salmonella from foods after preservation techniques.

Description:
Edel and Kampelmacher noted that food preservation techniques such as heat, desiccation, preservatives, high osmotic pressures or pH changes can cause sub-lethal injury Salmonella microorganisms. Preenrichment with Buffered Peptone Water allows for repair of cell damage by maintaining a high pH over the preenrichment period . The high pH capacity is especially useful for vegetable samples which have a low buffering capacity

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color


Directions: 1. Dissolve 20grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli ATCC 25922

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Yellow beige, clear, complete.

pH (25C): 7.20.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.