Technical Data
B8997-10
1,3-Butadiene, Conjugated
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Specificity:
Using a conjugate 1,3-Butadiene, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compound Crossreactivity ratio(a)
Butadiene-BSA 1
Acrolein-BSA 1/20,000
Formaldehyde-BSA 1/>50,000
Malondialdehyde-BSA 1/>50,000
Butanoic acid-BSA 1/>50,000
Propionic acid-BSA 1/>50,000

(a): 1,3-Butadiene-BSA concentration/conjugated close structure concnetration at half displacement.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 2 months at 4°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul-20°CBlue IceRabbit
Concentration:
Not Determined
Immunogen:
Synthetic 1,3-Butadiene conjugated to BSA.
Purity:
Purified by protein carrier (BSA) affinity column, and ammonium sulphate precipitation.
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile dH2O.
Specificity:
Recognizes 1,3-Butadiene conjugated with BSA. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.