Technical Data
C0010-01C
Complement C1q, Human
Description:
C1q is a sub-component of the first complement component C1, which is formed by the interaction of C1q with the tetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and is composed of three basic subunits termed A, B and C (28, 25, 24kD respectively) which asociate in the proportion 6 A-B subunits (52, 750D) and 3 C-C subunits (48kD), to form whole C1q (459kD). C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, which are also bound to C1q and so initiates the classical pathway of complement activation.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken-HRP Rb x Ch
I1906-12: IgY, Chicken-FITC Rb x Ch
I1906-14: IgY, Chicken-AP Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanChicken
Concentration:
~1mg/ml
Immunogen:
Full length human plasma C1q protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS. No preservatives added.
Specificity:
Recognizes human Complement C1q at ~26kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Leigh LE et al. C1q-mediated chemotaxis by human neutrophils: involvement of gClqR and G-protein signalling mechanisms. Biochem J 330 ( Pt 1):247-54 (1998).