C1q together with C1r and C1s composes the C1 macromolecule, the first component of the classical complement pathway. It functions as recognition unit by binding to immunoglobulins (IgM and IgG). C1q binding to IgG and IgM immune complexes activates C1r and C1s, which are bound to C1q and so initiates the classical pathway of complement activation.
Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry and Functional studies. Other applications have not been tested.
Immunohistochemistry (Frozen): 1:50
Optimal dilutions to be determined by the researcher.
Macrophages and follicular dendritic cells
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 0.1% BSA , 0.02% sodium azide|
|Recognizes mouse Complement C1q.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Zachrau, B et al; Antigen localization within the splenic marginal zone restores humoral immune response and IgG class switch in complement C4-deficient mice. Int Immunol 2004, 16: 1685. 2. Kang, Y et al; A Dominant Complement Fixation Pathway for Pneumococcal Polysaccharides Initiated by SIGN-R1 Interacting with C1q. Cell 2006, 125: 47. 3. Abbitt, K et al; Antibody ligation of murine Ly-6G induces neutropenia, blood flow cessation, and death via complement-dependent and independent mechanisms. J Leukoc Biol 2009, 85: 55. 4. Ankeny, D et al; B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice. J Clin Invest 2009, 119: 2990. 5. Suresh_m et al; Role of the property of C-Reactive Protein to activate the classical pathway of complement in protecting mice from pneumococcal infection. J Immunol 2006, 176: 4369|