Technical Data
C0010-06
Complement C1q, Human
Description:
C1q is serum glycoprotein of 18-polypeptides chains consisting of three non-identical subunits, A (29kD), B (26kD) and C (246aa, 19kD) in molar ratio of 1:2:2. Detection of C1q in serum, in serum-C1 and within immune complexes; inhibits hemolytic activity of C1 by binding to C1q in the liquid phase (antibody concentration necessary to inhibit 50% of hemolytic activity in the fluid phase is ~0.85ug/ml). With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5ug/ml. Useful for detection of C1q-bearing immune complexes in patients with rheumatic disorders.

Applications:
Suitable for use in ELISA, Western Blot and Inhibition experiments (e.g. hemolytic activity). Other applications not tested.

Recommended Dilution:
Western Blot: 1:10-1:50 (ECL method). Incubate for 1hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15F91Supernatant
SizeStorageShippingSourceHost
5ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified human complement C1q.
Purity:
Hybridoma culture supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes human Complement C1q. Polypeptide Reacting: C-chain of complement C1q (25kD in SDS-PAGE).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Heinz HP, Burger R, Golan DG, Loos M: Activation of the first component of complement, C1, by a monoclonal antibody recognizing the C chain of C1q. J Immunology 132, 804-808 (1984). 2. Antes U, Heinz HP, Loos M: Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies. J Immunol Methods 74, 299-306 (1984).
3. Antes U, Heinz HP, Loos M: Detection of C1q-bearing immune complexes by a monoclonal anti-C1q ELISA system. J Immunol Methods 102, 149-156 (1987).