Technical Data
C0010-07
Complement C1q, Human
Description:
C1q is serum glycoprotein of 18-polypeptides chains consisting of three non-identical subunits, A (29kD), B (26kD) and C (246aa, 19kD) in molar ratio of 1:2:2. C1Q in the plasma is complexed with two proenzymes C1r and two C1s molecules to form the first component of complement (C1). Activation of complement via classical pathway is triggered by binding of globular head of C1q to immune complexes containing IgG (Fc-region) or IgM or to a variety of other activating substances, including C-reactive protein, retrovirus and mitochondria. Subsequent to C1q binding, c1r and C1s are converted to proteolytic enzymes that are responsible to continuation of activation via the classical pathway. Normal plasma concentration of C1q is 100-180mg/L.

Applications:
Suitable for use in ELISA, Western Blot and Inhibition experiments (e.g. hemolytic activity). Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:100 (ECL method); Incubation Time 1hr at RT.
Inhibition of hemolytic activity: 0.85ug/ml inhibits 50% of hemolytic activity in the fluid phase. With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5ug/ml. Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F91Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Purified human complement C1q.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes the C-chain of human complement C1q (25kD in SDS-PAGE). Useful for detection of C1q-bearing immune complexes in patients with rheumatic disorders. Detects C1q in serum, in serum-C1 and within immune complexes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Heinz HP, Burger R, Golan DG, Loos M: Activation of the first component of complement, C1, by a monoclonal antibody recognizing the C chain of C1q. J Immunology 132, 804-808 (1984). 2. Antes U, Heinz HP, Loos M: Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies. J Immunol Methods 74, 299-306 (1984). 3. Antes U, Heinz HP, Loos M: Detection of C1q-bearing immune complexes by a monoclonal anti-C1q ELISA system. J Immunol Methods 102, 149-156 (1987).