Technical Data
C0013-01B
Bcr (ALL, BCR1, Breakpoint cluster region protein, CML, D22S11, D22S662, FLJ16453, PHL, Renal carcinoma antigen NY-REN-26)
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Technical Data |
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C0013-01B |
Bcr (ALL, BCR1, Breakpoint cluster region protein, CML, D22S11, D22S662, FLJ16453, PHL, Renal carcinoma antigen NY-REN-26) |
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Description: The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160kD protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:20 Western Blotting: 1:2,000-10,000 Immunohistochemistry: 1:50-100 Immunocytochemistry: 1:100-250 Immunoprecipitation: 1:60 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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