Technical Data
Bcr (ALL, BCR1, Breakpoint cluster region protein, CML, D22S11, D22S662, FLJ16453, PHL, Renal carcinoma antigen NY-REN-26)
The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160kD protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20
Western Blotting: 1:2,000-10,000
Immunohistochemistry: 1:50-100
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:60

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
A synthetic peptide corresponding to residues in the C-term of human Bcr
Supplied as a liquid
Recognizes human Bcr. Does not recognize mouse or rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Clark, S. S.,et al., (1988). Science 239, 775-777 2. Konopka, J. B.,et al.,(1984). Cell 37, 1035-1042 3. Boguski, M. al., (1993). Nature 366, 643-654. 4. Braselmann, S. and McCormick, F. (1995). EMBO J. 14, 4839-4848