Technical Data
C0013-01D
Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)
Description:
The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160kD protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).

Applications:
Suitable for use in Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:40

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Immunogen:
A synthetic peptide corresponding to residues in the C-term of human Bcr
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid
Specificity:
Recognizes human Bcr
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Clark, S. S., McLaughlin, J., Timmons, M., Pendergast, A. M., Ben-Neriah, Y., Dow, L. W., Crist, W., Rovera, G., Smith, S. D. and Witte, O. N. (1988). Science 239, 775-777 2. Konopka, J. B., Watanabe, S. M. and Witte, O. N. (1984). Cell 37, 1035-1042 3. Boguski, M. S. and McCormick, F. (1993). Nature 366, 643-654. 4. Braselmann, S. and McCormick, F. (1995). EMBO J. 14, 4839-4848