Technical Data
Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)
The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160kD protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).

Suitable for use in Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:40

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
A synthetic peptide corresponding to residues in the C-term of human Bcr
Purified by immunoaffinity chromatography.
Supplied as a liquid
Recognizes human Bcr
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Clark, S. S., McLaughlin, J., Timmons, M., Pendergast, A. M., Ben-Neriah, Y., Dow, L. W., Crist, W., Rovera, G., Smith, S. D. and Witte, O. N. (1988). Science 239, 775-777 2. Konopka, J. B., Watanabe, S. M. and Witte, O. N. (1984). Cell 37, 1035-1042 3. Boguski, M. S. and McCormick, F. (1993). Nature 366, 643-654. 4. Braselmann, S. and McCormick, F. (1995). EMBO J. 14, 4839-4848