Technical Data
C0013-26B
Cbl, CT
Description:
c-Cbl, product of c-cbl proto-oncogene, is a 120kD cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100
Western Blotting: 1:5,000
Immunocytochemistry: 1:100
Immunoprecipitation: 1:100

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k57Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Immunogen:
A synthetic peptide corresponding to residues in C-terminus of human Cbl
Purity:
Supernatant
Form
Supplied as a liquid
Specificity:
Recognizes human Cbl
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blake, T.J. et al. (1991) Oncogene 6, 653–657. 2. Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473–482. 3. Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294–307. 4. Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323–8331. 5. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335–339. 6. Hunter, S. et al. (1999) J. Biol. Chem. 274, 2097–2106.