Technical Data
C0013-26C
Cbl, phosphorylated, Tyr774
Description:
c-Cbl, product of c-cbl proto-oncogene, is a 120kD cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry:
Western Blotting: 1:1,000
Immunohistochemistry: 1:25
Immunocytochemistry: 1:25
Immunoprecipitation:

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k58Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr774 of human Cb
Purity:
Supernatant
Form
Supplied as a liquid
Specificity:
Recognizes human Cbl when phosphorylated at Tyr774.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langdon, W. Y., et al. Proc. Natl. Acad. Sci. USA 86: 1168–72 (1989). 2. Blake, T. J., et al.. EMBO J. 12: 2017–26 (1993). 3. Joazeiro, C. A., et al. Science 286: 309–12 (1999). 4. Galisteo, M. L., et al. J. Biol. Chem. 270: 20242–5 (1995).