Technical Data
C0013-31
Cbl, phosphorylated (Ser669) (Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55, RNF55, Signal transduction protein CBL)
Description:
Cbl was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
Mab10J92Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Phospho specific peptide corresponding to residues surrounding Serine 669 of human Cbl.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human Cbl at ~120kD only when phosphorylated at Serine 669. Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.