		Technical Data
C0013-32	Cbl-b (CBLb, Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b, Casitas B-lineage Lymphoma Proto-oncogene b, Nbla00127, RING Finger Protein 56, RNF56)

Description:
CBLB (Cas Br M (murine) ecotropic retroviral transforming sequence b) has high homology to CBL, the proto-oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. Like CBL, CBLB contains putative nuclear localization signal, zinc finger, leucine zipper, and proline-rich domains. CBLB associates with FYN, FGR, and PLCG1.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.2-2ug/ml
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	8C51	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Partial sequence of recombinant full-length protein to corresponding to the C-terminal region of human Cbl-b.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide before the addition of glycerol to 40%.

Specificity:

Recognizes human Cbl-b at ~109kD in HeLa cell lysate. Species Crossreactivity: mouse (NIH3T3 cells).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.