Technical Data
C0013-32A
Cbl (CBL, CBL2, C-CBL, RNF55, Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence, Oncogene CBL2)
Description:
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 14aa near the carboxy terminal of human cbl (P22681).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes the carboxy terminus of human Cbl. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thien, C.B.F., et al., (2005), "C-Cbl and Cbl-b ubiquitin ligases: substrate diversity and the negative regulation of signaling responses", Biochem. J., 391: 153-166. 2. Weissman, A.M., et al., (2001), "Themes and variations on ubiquitylation", Nat. Rev. Mol. Cell Biol., 2: 169-178. 3. Swaminathan, G., et al., (2006), "The Cbl family of proteins: ring leaders in regulation of cell signaling", J. Cell. Physiol., 209: 21-43.