Technical Data
C0031
C-MYC (PE)
Description:
The 62kD c-myc gene product is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.

Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: Neat. Use 10ul to label 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with spleen cells from Balb/c mice.

Recommended Negative Control:
I1904-79D: IgG1 Murine Negative Control (PE)

Storage and Stability:
Lyophilized powder may be stored at 4C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG19A133Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceMouse
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the sequence, AEEQKLISEEDLL at the C-terminal region of human c-myc.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human C-MYC. May be used to detect the commonly used c-myc tag.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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