Technical Data
C0045-61A
C3IP1 (KLHL12, C3IP1, DKIR, CUL3-interacting protein 1, Kelch-like 12)
Description:
Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3. Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: Predicted molecular weight 68kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanChicken
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide: KHAKKEREES LPNLLQYVRM PLLTPRYITD VIDAEPFIRC SLQCRDLVDE AKKFHLRPEL RSQMQGPRTR ARLGANEVLL VVGGFGSQQS PIDVVEKYDP KTQEWSFLPS ITRKRRYVAS VSLHDRIYVI GGYDGRSRLS SVECLDYTAD EDGVWYSVAP MNVRRGLAGA TTLGDMIYVS GGFDGSRRHT SMERYDPNID, corresponding t
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative.
Specificity:
Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with Xenopus laevis (92% identity with immunogen) due to sequence homology.Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.