Technical Data
C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced gene C4 protein, MIG-C4, UNQ491/PRO1007)
LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296aa GPI-linked glycoprotein. It contains two UPAR domains (aa33-126 and 140-222), and an ADAM cleavage site (aa312-325). The molecule shows extensive N- and O-linked glycosylation. There is one potential isoform that shows a deletion of aa129-180. Over aa 31-286, human LYPD3 shares 81% aa identity with mouse LYPD3.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (direct): 0.5-1.0ug/ml with the appropriate secondary reagents to detect human C4.4A. The detection limit for rhC4.4A is approximately 0.2ng/well.
Western Blot: 1.0ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hC4.4A.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
Recombinant corresponding to aa 31-286 human C4.4A expressed in NSO cells (O95274).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Recognizes human C4.4A. In direct ELISA, shows ~15% cross-reactivity with rmC4.4A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.