Technical Data
C0050-01D
CA125 Cancer Antigen (Mucin 16, MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125)
Description:
CA125/MUC16 is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000kD with smaller 'subunits' being described by some investigators.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-5ug/ml after electrophoresing 20ug of sample.
Immunohistochemistry: 1-5ug/ml
Note: Dilute in PBS or medium which is identical to that used in the assay system.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J453Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
CA-125 purified from human ovarian carcinoma. Cellular Localization: Cell Membrane
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in 10mM Tris, pH 7.5, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human ovarian carcinoma cells. Specifically recognizes epitope specificity group B (ISOBM classification).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Heinzelmann-Schwarz VA et al. Clin Cancer Res 10:4427-36 (2004).